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By Leonard G. Davis (auth.), John M. Pezzuto Ph.D., Michael E. Johnson Ph.D., Henri R. Manasse Jr. Ph.D. (eds.)

Biotechnology and Pharmacy deals a distinct review of the foundations of biotechnology and their purposes within the pharmaceutical sciences. The publication assumes a easy wisdom of biology and chemistry and used to be written as a textual content compatible for college students of pharmacy or different well-being sciences. the 1st a part of the publication describes the elemental components of biotechnology, corresponding to recombinant DNA and monoclonal antibody expertise; the second one half comprehensively covers purposes of biotechnology within the prognosis and remedy of sickness; and the ultimate half deals a realistic dialogue of ways biotechnology items will impact the perform of pharmacy.
Microbiologists, biochemists, and medicinal chemists also will locate this booklet to be a precious reference.

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42. , and Nakanishi, S. 1987. cDNA cloning of bovine substance-K receptor through oocyte expression system. Nature (London) 329:836-838. 43. , and Schwartz, LC. 1990. Molecular cloning and characterization of a novel dopamine receptor as a target for neuroleptics. Nature (London) 347:146-151. 44. , et al. 1984. Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence. Nature (London) 312:121-127. 45. , et al. 1987. Primary structure of the receptor for calcium channel blockers from skeletal muscle.

22 Background to Recombinant DNA Technology I 23 complementary to the sequence of interest. Although longer cDNA (and cRNA) probes have been used successfully, we have used synthetic oligodeoxyribonucleotide (oligonucleotide) probes,63-65 since they offer a number of advantages over cDNA probes which include the following: (1) they allow the use of published sequences, which alleviates the need to clone the mRNA of interest as well as the task of implementing all the requisite microbiological techniques; (2) they can be designed to distinguish two homologous mRNAs from the same gene family; and (3) they can be designed to be complementary to specific regions of a given mRNA to study differential utilization of exons in various cell types.

38. S. 1983. Isolation of a cDNA clone for the type I regulatory subunit of bovine cAMP-dependent protein kinase. Proc. Natl. Acad. Sci. A. 80:3608-3612. 39. , et al. 1984. Expression offunctional acetylcholine receptor from cloned cDNAs. Nature (London) 307:604-608. 40. , et al. 1987. cDNA for the human b2-adrenergic receptor: A protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor.

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